Reporter

Part:BBa_K1634006:Design

Designed by: Xinhong Chen   Group: iGEM15_China_Tongji   (2015-09-14)


dsRed ( a reporter which is a red fluorescent protein)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 478


Design Notes

Since we use the restriction enzyme site to do the ligation, we should pay attention to the sequence of dsRed, avoiding using the restriction site exits in it.


Source

DsRed is a commonly used reporter,so we get it from some plamids by PCR.

References